Florence Delépine studied music at the Tours Regional National Conservatoire, where she was awarded First Prizes in chamber music, sight-reading and flute. She went on to study at the Paris National Conservatoire of Music and Dance (CNSMDP), where she worked with Michel Debost and Pierre-Yves Artaud. She was awarded First Prize in chamber music (first category) and First Prize in flute.

Since 1990, she has been principal flute with the Orchestre de Paris.

In 1994, she obtained the Certificate of Aptitude for the role of flute teacher and joined the Paris National Opera as a principal flutist, a post she left a year later.

She taught at the CNSMDP as an assistant to Pierre-Yves Artaud until 2014, and subsequently to Philippe Bernold.